Twenty specimens were collected from some Iraqi hospitals and diagnosed according to morphological characteristics and biochemical tests. The antimicrobial of silver and copper nanoparticles were recently focused on studied in the literature. In this article experiments were done to see the effect of silver and copper nanoparticles on grams negative and gram positive bacteria. The effect of both nanoparticles was higher in Pseudomonas aerugenosa and Klebsiella pneumoniae than Streptococcus pyogenes and the effect of two nanobartical are more effective than nanoparticles alone.